Associate Scientist - Oncology

职位描述

The Oncology department at Gilead Sciences is seeking an experienced and motivated Associate Scientist with exceptional technical skills to support research programs focused on the discovery and development of novel therapies that mobilize the immune response against cancer. The successful candidate will be instrumental in leading cell engineering efforts, including generation of genetically modified cell lines and conducting experiments and help develop novel assays to test therapeutic hypotheses and advance immune engager therapeutic candidates to selectively target tumors. Candidate will also develop novel assays and conduct experiments to test therapeutic hypotheses and advance immune engager therapeutic candidates to selectively target tumors.

Our highly diverse, collaborative, and interdisciplinary research teams utilize state-of-the-art technologies and innovative methodologies to advance novel immuno-oncology therapies into the clinic. The position has ample opportunities for career growth through acquisition of new skills, experiences, and professional relationships in an inclusive, fast-paced, and resource-rich environment. The successful candidate will contribute to therapeutic candidate characterization in immune cell-mediated killing assays, new admixed immune cells / cancer cells assay development, mechanism of action studies, and flow cytometry-based immune profiling/phenotyping studies in vitro and from dissociated mouse tumor samples.

Key Responsibilities

• Primary responsibility to support target validation efforts through characterization and generation of cellular models, including genetically modified cell lines (CRISPR knock-out) and overexpressing cell lines, with contributions to multiple projects

• Explore mechanism of action of immune engager molecules by characterizing mouse surrogate molecules, developing genetically engineered mouse cancer cell lines

• Help develop novel, complex cell biology assays to model tumor biology more physiologically than traditional cell culture systems (organoid, sequential killing assays)

• Prepare and present findings to the team and at department-wide meetings

• Contribute figures to research reports, manuscripts, INDs, and patent filings

Knowledge, Experience and Skills

• Industry research experience preferred (BS +4 years OR MS +2 years) in cancer immunology or related field with a record of achievements (publications)

• Proficiency with cell culture methods

• Experience with gene knockdown (RNAi), knockout (CRISPR/Cas9), and overexpression techniques (transfection, viral transduction)

• Experience with RNA and protein quantification techniques (RT-qPCR, Nanostring, MSD/ELISA, western)

• Basic flow cytometry skills, proficiency with multi-parametric Flow Cytometry experiments preferred

• Experience in running and developing cell-based assays (ADCC or T cell-mediated killing assays, Jurkat reporter assays, etc…)

• Independently execute and analyze studies

• Great attention to detail with excellent organizational and record keeping skills

• Strong motivation, independence, and ability to think critically and act creatively to resolve problems

• Excellent communication and interpersonal skills and ability to integrate into a high-paced multidisciplinary environment